In Vitro Guide: Tissue Culture Protocols for Houseplants & Ornamentals – Book 2: 50 Advanced Protocols for Palms, Ferns, Succulents & More Paperback – September 28, 2025

Following the success of In Vitro Guide: Tissue Culture Protocols for Houseplants & Ornamentals (Book 1), this second volume delivers 50 more complete micropropagation recipes, covering high-demand species from velvet-leaf philodendrons and rare anthuriums to fan palms, ferns, succulents, and cacti.Whether you’re a collector safeguarding prized genetics, a hobbyist exploring sterile culture at home, or an entrepreneur building a compact lab, this book is your hands-on reference for cloning today’s most beautiful and valuable plants.You’ll learn the process step by step—from preparing explants to mixing media, balancing hormones, preventing contamination, and acclimating plantlets. Each protocol is written as a recipe with exact measurements, sterilization times, hormone levels, and acclimatization tips—so you can follow along in a home setup or scale in a nursery.Inside you’ll learn how to:✔ Prepare sterile explants from nodes, leaves, rhizomes, or spores✔ Mix Murashige & Skoog (MS) medium with sucrose, agar, and PGRs✔ Apply species-specific hormone balances to multiply shoots✔ Root plantlets and transfer them successfully into soil mixes✔ Prevent contamination, vitrification, and callus overgrowth✔ Build a reliable workspace with a still air box or flow hood✔ Scale your culture workflow from jars on a shelf to nursery runs✅ Includes exact protocols for 50 ornamentals:Philodendron & Monstera: P. verrucosum, P. rugosum, P. sodiroi, P. plowmanii, P. nangaritense, P. mexicanum, P. luxurians, P. atabapoense, P. gloriosum (Dark Form), Monstera pinnatipartitaAlocasia: A. zebrina, A. reginula ‘Black Velvet’, A. odora, A. micholitziana ‘Frydek Variegated’, A. macrorrhizosAnthurium: A. crystallinum, A. magnificum, A. veitchii, A. forgetii, A. villenaorumPrayer Plants: Calathea warscewiczii, C. musaica ‘Network’, C. crocata, C. ornata, C. louisae ‘Maui Queen’, C. lietzei ‘White Fusion’, C. picturata, Maranta leuconeura erythroneura, M. kerchoveana, Stromanthe sanguinea ‘Triostar’Ferns: Microsorum musifolium ‘Crocodile’, Platycerium bifurcatum (two methods), Blechnum gibbum ‘Silver Lady’, Davallia fejeensis, Pteris creticaPalms: Licuala grandis, Chamaedorea seifrizii, Rhapis excelsa, Phoenix roebelenii, Howea forsterianaSucculents & Cacti: Agave americana (two methods), Gasteria bicolor, Sansevieria trifasciata, Kalanchoe tomentosa, Sedum morganianum, Lithops spp., Astrophytum myriostigma, Gymnocalycium mihanovichii, Rebutia heliosaEuphorbia: Euphorbia trigonaWho this book is for:Rare plant collectors preserving elite geneticsHobbyists eager to try professional propagation at homeNurseries seeking uniform, pest-free productionEntrepreneurs testing micropropagation startupsEducators and students in plant biotechnologyEach protocol is formatted for clarity and quick reference—with hormone dosages, sterilization timings, media composition, and acclimatization notes. Whether you’re working with a pressure cooker in your kitchen or a laminar flow hood, you’ll have everything you need.With 50 protocols spanning aroids, prayer plants, anthuriums, alocasias, ferns, palms, succulents, cacti, and euphorbia, this second volume of the In Vitro Plant Lab Series equips you to culture confidently, troubleshoot effectively, and expand your plant collection like never before.Grow clean. Grow fast. Grow smart. One sterile cup at a time. Read more